Peer Reviewed

1

Document Type

Article

Publication Date

13-2-2019

Keywords

A549 Cells, Anti-Inflammatory Agents, Cell Survival, Collagen, Drug Carriers, Glycerophosphates, Humans, Hydrogels, Immunologic Factors, Immunomodulation, Interleukins, Lipids, Mesenchymal Stem Cell Transplantation, Methylcellulose, Nanoparticles, Pulmonary Disease, Chronic Obstructive, Signal Transduction, Tretinoin

Funder/Sponsor

School of Pharmacy, Royal College of Surgeons in Ireland. Science Foundation Ireland (SFI) [Investigator Award 13/IA/840]

Comments

This is a pre-print of an article published in Pharmaceutical Research. The final authenticated version is available online at: DOI:10.1007/s11095-019-2583-x

Abstract

PURPOSE: To investigate two potential strategies aimed at targeting the inflammatory pathogenesis of COPD: a small molecule, all trans retinoic acid (atRA) and human mesenchymal stem cells (hMSCs).

METHODS: atRA was formulated into solid lipid nanoparticles (SLNs) via the emulsification-ultrasonication method, and these SLNs were characterised physicochemically. Assessment of the immunomodulatory effects of atRA-SLNs on A549 cells in vitro was determined using ELISA. hMSCs were suspended in a previously developed methylcellulose, collagen and beta-glycerophosphate hydrogel prior to investigating their immunomodulatory effects in vitro.

RESULTS: SLNs provided significant encapsulation of atRA and also sustained its release over 72 h. A549 cells were viable following the addition of atRA SLNs and showed a reduction in IL-6 and IL-8 levels. A549 cells also remained viable following addition of the hMSC/hydrogel formulation - however, this formulation resulted in increased levels of IL-6 and IL-8, indicating a potentially pro-inflammatory effect.

CONCLUSION: Both atRA SLNs and hMSCs show potential for modulating the environment in inflammatory disease, though through different mechanisms and leading to different outcomes - despite both being explored as strategies for use in inflammatory disease. atRA shows promise by acting in a directly anti-inflammatory manner, whereas further research into the exact mechanisms and behaviours of hMSCs in inflammatory diseases is required.

Disciplines

Pharmacy and Pharmaceutical Sciences | Translational Medical Research

Citation

Payne CM, Burke LP, Cavanagh B, O'Toole D, Cryan SA, Kelly HM. Evaluation of the Immunomodulatory Effects of All-Trans Retinoic Acid Solid Lipid Nanoparticles and Human Mesenchymal Stem Cells in an A549 Epithelial Cell Line Model. Pharmaceutical Research. 2019;36(4):50

PubMed ID

30761406

DOI Link

10.1007/s11095-019-2583-x

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

Available for download on Thursday, February 13, 2020

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