Peer Reviewed

1

Document Type

Article

Publication Date

2015

Keywords

Silica Nano-Particles, Polymer Brushes, ARGET ATRP, Poly(methacrylic acid), Bio-Conjugation, Enzyme Immobilization

Funder/Sponsor

This work was supported by funding from Science Foundation Ireland (07/IN1/B1792). Marcello Iacono gratefully acknowledges support from the Biomedical Diagnostic Institute (BDI) and the Irish Separation Science Cluster (ISSC).

Comments

This article is also available at http://www.mdpi.com/2073-4360/7/8/1427

Abstract

The synthesis of polymer brush decorated silica nano-particles is demonstrated by activator regeneration by electron transfer atom transfer radical polymerization (ARGET ATRP) grafting of poly(tert-butyl methacrylate). ATRP initiator decorated silica nano-particles were obtained using a novel trimethylsiloxane derivatised ATRP initiator obtained by click chemistry. Comparison of de-grafted polymers with polymer obtained from a sacrificial initiator demonstrated good agreement up to 55% monomer conversion. Subsequent mild deprotection of the tert-butyl ester groups using phosphoric acid yielded highly colloidal and pH stable hydrophilic nano-particles comprising approximately 50% methacrylic acid groups. The successful bio-conjugation was achieved by immobilization of Horseradish Peroxidase to the polymer brush decorated nano-particles and the enzyme activity demonstrated in a conversion of o-phenylene diamine dihydrochloride assay.

Disciplines

Pharmacy and Pharmaceutical Sciences

Citation

Iacono M, Heise A. Stable Poly(methacrylic acid) Brush Decorated Silica Nano-Particles by ARGET ATRP for Bioconjugation. Polymers. 2015;7:1427-1443.

DOI Link

10.3390/polym7081427

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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