Title
Screening of siRNA Nanoparticles for Delivery to Airway Epithelial Cells Using High Content Analysis
Peer Reviewed
1
Document Type
Article
Publication Date
8-2011
Keywords
High Content Analysis, RNAi, PEI, Mitochondrial Membrane Potential
Abstract
Background:
Delivery of siRNA to the lungs via inhalation offers a unique opportunity to develop novel methods of treating a range of poorly treated respiratory conditions. However progress has been greatly hindered by safety and delivery issues. This study developed a high-throughput method for screening novel nanotechnologies for pulmonary siRNA delivery
Methodology:
Following physico-chemical analysis, the ability of PEI-PEG/siRNA nanoparticles to facilitate siRNA delivery was determined using high content analysis (HCA) in Calu-3 cells. Results obtained from HCA were validated using confocal microscopy. Finally, cytotoxicity of the PEI-PEG/siRNA particles was analysed by HCA using the Cellomics® multiparamter cytotoxicity assay.
Conclusions:
PEI-PEG/siRNA nanoparticles facilitated increased siRNA uptake and luciferase knockdown in Calu-3 cells compared to PEI/siRNA.
Disciplines
Pharmacy and Pharmaceutical Sciences
Citation
Hibbitts A, Lieggi N, McCabe O, Thomas W, Barlow J, O’Brien F, Cryan SA. Screening of siRNA Nanoparticles for Delivery to Airway Epithelial Cells Using High Content Analysis. Therapeutic Delivery. 2011;2(8):987-999.
Link to this item at
http://epubs.rcsi.ie/spharmart/4/
DOI Link
10.4155/tde.11.73
Comments
This article is also availble at http://www.future-science.com/doi/abs/10.4155/tde.11.73