Peer Reviewed

1

Document Type

Article

Publication Date

11-5-2017

Keywords

microRNA-134, epilepsy biomarkers

Funder/Sponsor

This material is based upon works supported by the SFI/TIDA under Project No. 14/TIDA/2356 (to ES) and cofunded by Enterprise Ireland and the ERDF under Award CF-2016-0552-P (to RJF). Support for this research was also from Science Foundation Ireland (SFI) under grants SFI/13/IA/1891, SFI/14/ADV/RC2721 (to DCH), 13/ SIRG/2114 (to E.J.-M.), the European Union’s ‘Seventh Framework’ Programme (FP7) under Grant Agreement no. 602130, the Detlev-Wrobel-Fonds for Epilepsy Research Frankfurt (to F.R.) and a fellowship from the Iraqi Ministry of Higher Education and Scientific Research (to R.R.).

Comments

The original article is available at www.nature.com

Abstract

Diagnosis of seizure disorders such as epilepsy currently relies on clinical examination and electroencephalogram recordings and is associated with substantial mis-diagnosis. The miRNA, miR-134 (MIR134 in humans), has been found to be elevated in brain tissue after experimental status epilepticus and in human epilepsy cells and their detection in biofluids may serve as unique biomarkers. miRNAs from unprocessed human plasma and human cerebrospinal fluid samples were used in a novel electrochemical detection based on electrocatalytic platinum nanoparticles inside a centrifugal microfluidic device where the sandwich assay is formed using an event triggered release system, suitable for the rapid point-of-care detection of low abundance biomarkers of disease. The device has the advantage of controlling the rotation speed of the centrifugal device to pump nanoliter volumes of fluid at a set time and manipulate the transfer of liquids within the device. The centrifugal platform improves reaction rates and yields by proposing efficient mixing strategies to overcome diffusion-limited processes and improve mass transport rates, resulting in reduced hybridization times with a limit of detection of 1 pM target concentration. Plasma and cerebrospinal fluid samples (unprocessed) from patients with epilepsy or who experienced status epilepticus were tested and the catalytic response obtained was in range of the calibration plot. This study demonstrates a rapid and simple detection for epilepsy biomarkers in biofluid.

Disciplines

Physics | Physiology

Citation

McArdle H, Jimenez-Mateos E, Raoof R, Carthy E, Boyle D, ElNaggar H, Delanty N, Hamer H, Dogan M, Huchtemann T, Krtvelyessy P, Rosenow F, Forster RJ, Henshall DC, Spain E. "TORNADO" - Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid. Scientific Reports. 2017; 7(1):1750

PubMed ID

28496112

DOI Link

10.1038/s41598-017-01947-2

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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