Authors

Niamh M. Connolly, Royal College of Surgeons in IrelandFollow
Pierre Theurey, University of Padova
Vera Adam-Vizi, Semmelweis Univeristy
Nicolas G. Bazan, Louisiana State University Health New Orleans
Paolo Bernardi, University of Padova
Juan P. Bolaños, University of Salamanca
Carsten Culmsee, University of Marburg
Valina L. Dawson, John Hopkins University
Mohanish Deshmukh, University of North Carolina
Michael R. Duchen, University College London
Heiko Düssmann, Royal College of Surgeons in IrelandFollow
Gary Fiskum, University of Maryland School of Medicine
Maria F. Galindo, Universitario de Albacete
Giles E. Hardingham, Edinburgh Medical School
J Marie Hardwick, John Hopkins University
Mika B. Jekabsons, University of Mississippi
Elizabeth A. Jonas, Yale University
Joaquin Jordán, University of Castilla-La Mancha
Stuart A. Lipton, University of California San Diego
Giovanni Manfredi, Weill Cornell Medicine
Mark P. Mattson, National Institute on Aging Intramural Research Program
BethAnn McLaughlin, Vanderbilt University School of Medicine
Axel Methner, University Medical Centre Mainz
Anne N. Murphy, University of California
Michael P. Murphy, University of Cambridge
David G. Nicholls, Buck Institute for Research on Aging
Brian M. Polster, University of Maryland School of Medicine
Tullio Pozzan, University of Padova
Rosario Rizzuto, University of Padova
Jorgina Satrústegui, Universidad Autonoma de Madrid
Ruth S. Slack, University of Ottawa
Raymond A. Swanson, University of California, San Francisco
Russell H. Swerdlow, University of Kansas
Yvonne Will, Pfizer
Zheng Ying, Soochow University
Alvin Joselin, University of Ottawa
Anna Gioran, German Center for Neurodegenerative Diseases
Catarina Moreira Pinho, Karolinska Institutet
Orla Watters, Royal College of Surgeons in IrelandFollow
Manuela Salvucci, Royal College of Surgeons in IrelandFollow
Irene Llorente-Folch, Royal College of Surgeons in IrelandFollow
David S. Park, University of Ottawa
Daniele Bano, German Center for Neurodegenerative Diseases
Maria Ankarcrona, Karolinska Institute
Paola Pizzo, University of Padova
Jochen HM Prehn, Royal College of Surgeons in IrelandFollow

Peer Reviewed

1

Document Type

Article

Publication Date

11-12-2017

Keywords

Mitochondrial dysfunction, Neurodegenerative disease

Funder/Sponsor

We acknowledge the support of the CeBioND EU Joint Programme for Neurodegenerative Disease Research (JPND; www.jpnd.eu). The programme is supported through the following national funding organisations: Canada, CIHR; Germany, BMBF; Ireland: Science Foundation Ireland (14/JPND/ B3077); Italy: MIUR; Sweden: VR.

Comments

This article is also available at https://www.nature.com/articles/s41418-017-0020-4

Abstract

Neurodegenerative diseases are a spectrum of chronic, debilitating disorders characterised by the progressive degeneration and death of neurons. Mitochondrial dysfunction has been implicated in most neurodegenerative diseases, but in many instances it is unclear whether such dysfunction is a cause or an effect of the underlying pathology, and whether it represents a viable therapeutic target. It is therefore imperative to utilise and optimise cellular models and experimental techniques appropriate to determine the contribution of mitochondrial dysfunction to neurodegenerative disease phenotypes. In this consensus article, we collate details on and discuss pitfalls of existing experimental approaches to assess mitochondrial function in in vitro cellular models of neurodegenerative diseases, including specific protocols for the measurement of oxygen consumption rate in primary neuron cultures, and single-neuron, time-lapse fluorescence imaging of the mitochondrial membrane potential and mitochondrial NAD(P)H. As part of the Cellular Bioenergetics of Neurodegenerative Diseases (CeBioND) consortium ( www.cebiond.org ), we are performing cross-disease analyses to identify common and distinct molecular mechanisms involved in mitochondrial bioenergetic dysfunction in cellular models of Alzheimer's, Parkinson's, and Huntington's diseases. Here we provide detailed guidelines and protocols as standardised across the five collaborating laboratories of the CeBioND consortium, with additional contributions from other experts in the field.

Disciplines

Physics | Physiology

Citation

Connolly NMC, Theurey P, Adam-Vizi, V, Bazan NG, Bernardi P, Bolanos JP, et al. Cell Death & Differentiation 2017[Epub ahead of print]

PubMed ID

29229998

DOI Link

10.1038/s41418-017-0020-4

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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