Peer Reviewed
1
Document Type
Article
Publication Date
6-2010
Keywords
Bacterial Proteins, Blotting, Western, Chromatography, High Pressure Liquid, Elafin, Electrophoresis, Polyacrylamide Gel, Fibronectins, Humans, Mass Spectrometry, Metalloproteases, Pseudomonas aeruginosa
Abstract
Elafin is a 6-kDa innate immune protein present at several epithelial surfaces including the pulmonary epithelium. It is a canonical protease inhibitor of two neutrophil serine proteases [neutrophil elastase (NE) and proteinase 3] with the capacity to covalently bind extracellular matrix proteins by transglutamination. In addition to these properties, elafin also possesses antimicrobial and immunomodulatory activities. The aim of the present study was to investigate the effect of Pseudomonas aeruginosa proteases on elafin function. We found that P. aeruginosa PAO1-conditioned medium and two purified Pseudomonas metalloproteases, pseudolysin (elastase) and aeruginolysin (alkaline protease), are able to cleave recombinant elafin. Pseudolysin was shown to inactivate the anti-NE activity of elafin by cleaving its protease-binding loop. Interestingly, antibacterial properties of elafin against PAO1 were found to be unaffected after pseudolysin treatment. In contrast to pseudolysin, aeruginolysin failed to inactivate the inhibitory properties of elafin against NE. Aeruginolysin cleaves elafin at the amino-terminal Lys6-Gly7 peptide bond, resulting in a decreased ability to covalently bind purified fibronectin following transglutaminase activity. In conclusion, this study provides evidence that elafin is susceptible to proteolytic cleavage at alternative sites by P. aeruginosa metalloproteinases, which can affect different biological functions of elafin.
Disciplines
Medicine and Health Sciences
Citation
Guyot N, Bergsson G, Butler MW, Greene CM, Weldon S, Kessler E, Levine RL, O'Neill SJ, Taggart CC, McElvaney NG. Functional study of elafin cleaved by Pseudomonas aeruginosa metalloproteinases. Biol Chem. 2010;391(6):705-16.
PubMed ID
20370321
Link to this item at
http://epubs.rcsi.ie/medart/28
DOI Link
10.1515/BC.2010.0XX
Comments
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