Algorithms, Amino Acid Sequence, Animals, Cell Differentiation, Chickens, Computational Biology, Humans, Molecular Sequence Data, Muscle, Skeletal, Myosin Heavy Chains, Phosphorylation, Phosphotyrosine, Phylogeny, Sequence Homology, Amino Acid
BACKGROUND: Previously it has been shown that insulin-mediated tyrosine phosphorylation of myosin heavy chain is concomitant with enhanced association of C-terminal SRC kinase during skeletal muscle differentiation. We sought to identify putative site(s) for this phosphorylation event.
RESULTS: A combined bioinformatics approach of motif prediction and evolutionary and structural analyses identified tyrosines163 and 1856 of the skeletal muscle heavy chain as the leading candidate for the sites of insulin-mediated tyrosine phosphorylation.
CONCLUSION: Our work is suggestive that tyrosine phosphorylation of myosin heavy chain, whether in skeletal muscle or in platelets, is a significant event that may initiate cytoskeletal reorganization of muscle cells and platelets. Our studies provide a good starting point for further functional analysis of MHC phosphor-signalling events within different cells.
Harney DF, Butler RK, Edwards RJ. Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach. Theoretical Biology & Medical Modelling. 2005;2:12.