Peer Reviewed

1

Document Type

Article

Publication Date

1-5-2018

Keywords

3D single-molecule localization microscopy, Point spread functions

Funder/Sponsor

U01 EB021223/EB/NIBIB NIH HHS/United States

Comments

The original publication is at www.nature.com

Abstract

We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves minimal uncertainty in 3D on any microscope and is compatible with any PSF engineering approach. We used this method to image cellular structures and attained unprecedented image quality for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D super-resolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics.

Disciplines

Life Sciences

Citation

Li Y, Mund M, Hoess P, Deschamps J, Matti U, Nijmeijer B, Sabinina VJ, Ellenberg J, Schoen I, Ries J. Real-time 3D single-molecule localization using experimental point spread functions. Nature Methods. 2018; 15(5):367-369

PubMed ID

29630062

DOI Link

29630062

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

Available for download on Wednesday, October 10, 2018

Included in

Life Sciences Commons

Share

COinS