Peer Reviewed

1

Document Type

Article

Publication Date

20-4-2018

Keywords

Staphylococcus Aureus, MRSA, antimicrobial resistance, coagulase negative staphylococci. nasal colonization.

Funder/Sponsor

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil under the Science without Borders Program (Grant number 9172-13-0)

Comments

The original article is available at http://bmjopen.bmj.com

Abstract

OBJECTIVE:

The aim of this study was to investigate co-located nasal Staphylococcus aureus and coagulase-negative staphylococci (CoNS) (mainly Staphylococcus epidermidis), recovered from healthy medical students in their preclinical year, prior to exposure to the healthcare environment, for the carriage of genes and genetic elements common to both species and that may contribute to S. aureus and methicillin-resistant S. aureus (MRSA) evolution.

DESIGN:

Prospective observational cross-sectional study. Carriage of antimicrobial resistance and virulence-associated genes in the absence of significant antibiotic selective pressure was investigated among healthy medical students from geographically diverse origins who were nasally co-colonised with S. aureus and CoNS. Clonal lineages of S. aureus isolates were determined.

SETTING/PARTICIPANTS:

Dublin-based international undergraduate medical students.

RESULTS:

Nasal S. aureus carriage was identified in 137/444 (30.8%) students of whom nine (6.6%) carried MRSA (ST59-MRSA-IV (6/9), CC1-MRSA-V-SCCfus (3/9)). The genes mecA, fusB, ileS2, qacA/qacC and the arginine catabolic mobile element-arc were detected among colonising nasal staphylococci and had a significantly greater association with CoNS than S. aureus. The rate of co-carriage of any of these genes in S. aureus/CoNS pairs recovered from the same individual was <1%.

CONCLUSIONS:

The relatively high prevalence of these genes among CoNS of the healthy human flora in the absence of significant antibiotic selective pressure is of interest. Further research is required to determine what factors are involved and whether these are modifiable to help prevent the emergence and spread of antibiotic resistance among staphylococci.

Disciplines

Medicine and Health Sciences

Citation

Budri PE, Shore AC, Coleman DC, Kinnevey PM, Humpreys H, Fitzgerald-Hughes D. Observational cross-sectional study of nasal staphylococcal species of medical students of diverse geographical origin, prior to healthcare exposure: prevalence of SCCmec, fusC, fusB and the arginine catabolite mobile element (ACME) in the absence of selective antibiotic pressure. BMJ Open. 2018;8(4):e020391

PubMed ID

29678979

DOI Link

10.1136/bmjopen-2017-020391 Free PMC Article

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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