Acid Phosphatase, Animals, Bone Morphogenetic Proteins, Bone and Bones, Cell Death, Collagen, Diphosphonates, Drug Delivery Systems, Durapatite, Fluorescent Dyes, Humans, Isoenzymes, Male, Mice, Osteoclasts, Osteogenesis, Porosity, Prosthesis Implantation, Rats, Rats, Wistar, Recombinant Proteins, Tissue Scaffolds, X-Ray Microtomography
Australian National Health Medical Research Council (NHMRC), Cancer Institute New South Wales Research Equipment, Ian Potter Foundation, Perpetual Foundation, Ramaciotti Foundation, Sydney Medical School Research Infrastructure Major Equipment Scheme.
An emerging paradigm in orthopedics is that a bone-healing outcome is the product of the anabolic (bone-forming) and catabolic (bone-resorbing) outcomes. Recently, surgical and tissue engineering strategies have emerged that combine recombinant human bone morphogenetic proteins (rhBMPs) and bisphosphonates (BPs) in order to maximize anabolism and minimize catabolism. Collagen-based scaffolds that are the current surgical standard can bind rhBMPs, but not BPs. We hypothesized that a biomimetic collagen-hydroxyapatite (CHA) scaffold would bind both agents and produce superior in vivo outcomes. Consistent with this concept, in vitro elution studies utilizing rhBMP-2 ELISA assays and scintillation counting of (14)C-radiolabeled zoledronic acid (ZA) confirmed delayed release of both agents from the CHA scaffold. Next, scaffolds were tested for their capacity to form ectopic bone after surgical implantation into the rat hind limb. Using CHA, a significant 6-fold increase in bone volume was seen in rhBMP-2/ZA groups compared to rhBMP-2 alone, confirming the ability of ZA to enhance rhBMP-2 bone formation. CHA scaffolds were found to be capable of generating mineralized tissue in the absence of rhBMP-2. This study has implications for future clinical treatments of critical bone defects. It demonstrates the relative advantages of co-delivering anabolic and anti-catabolic agents using a multicomponent scaffold system.
Murphy CM, Schindeler A, Gleeson JP, Yu NY, Cantrill LC, Mikulec K, Peacock L, O'Brien FJ, Little DG. A collagen-hydroxyapatite scaffold allows for binding and co-delivery of recombinant bone morphogenetic proteins and bisphosphonates. Acta Biomaterialia. 2014 May;10(5):2250-8
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