Peer Reviewed

1

Document Type

Article

Publication Date

30-12-2014

Keywords

Liposomes, Nanoparticles, Nanotoxicology, High Content Analysis, High Content Screening

Funder/Sponsor

Irish Health Research Board (HRB) HRB RP/2005/117 and PHD/2007/11.

Comments

This article is also available at http://www.tandfonline.com/doi/full/10.3109/08982104.2014.987785

Abstract

Carriers are often an essential element of drug delivery, bestowing attributes to their cargo such as biocompatibility, enhanced delivery, extended half-life and efficacy as well as mediating specific targeting at a tissue, cell or intracellular level. Liposomes and lipid-based carriers have been investigated for decades for this purpose, many achieving clinical approval including products such as Doxil® and Myocet™. Large-scale compound screens are routinely carried out in the field of drug discovery; however, less work has been done on harnessing high-throughput methods for carrier material screening. Screening the interaction of drug carriers and materials with cells is particularly critical for the development of emerging therapies, including biomedicines, in order to facilitate the development of safe and efficient drug products. Herein, a range of liposomes of neutral, anionic and cationic charge and others that are surface-modified with mannose residues were screened for cell interaction, toxicity and immune reactivity in THP-1-derived macrophages using a high-throughput format. Liposomes were seen to be efficacious in a concentration-dependent and, for mannosylated liposomes, mannosylated cholesterol linker length-dependent manner.

Disciplines

Pharmacy and Pharmaceutical Sciences

Citation

Kelly C, Lawlor C, Burke C, Barlow JW, Ramsey JM, Jefferies C, Cryan SA. High-throughput methods for screening liposome-macrophage cell interaction. Journal of Liposome Research. 2015;25(3):211-221.

PubMed ID

25547801

DOI Link

10.3109/08982104.2014.987785

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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