Peer Reviewed

1

Document Type

Article

Publication Date

3-2-2016

Keywords

Bioenergy, calcium cell level, calcium transport, cell stress, controlled study, enzyme activity, glucose transport, mathematical model, neuroprotection, sensitivity analysis.

Funder/Sponsor

Science Foundation Ireland. Health Research Board

Comments

The original article is available at http://journals.plos.org/

Abstract

Loss of ionic homeostasis during excitotoxic stress depletes ATP levels and activates the AMP-activated protein kinase (AMPK), re-establishing energy production by increased expression of glucose transporters on the plasma membrane. Here, we develop a computational model to test whether this AMPK-mediated glucose import can rapidly restore ATP levels following a transient excitotoxic insult. We demonstrate that a highly compact model, comprising a minimal set of critical reactions, can closely resemble the rapid dynamics and cell-to-cell heterogeneity of ATP levels and AMPK activity, as confirmed by single-cell fluorescence microscopy in rat primary cerebellar neurons exposed to glutamate excitotoxicity. The model further correctly predicted an excitotoxicity-induced elevation of intracellular glucose, and well resembled the delayed recovery and cell-to-cell heterogeneity of experimentally measured glucose dynamics. The model also predicted necrotic bioenergetic collapse and altered calcium dynamics following more severe excitotoxic insults. In conclusion, our data suggest that a minimal set of critical reactions may determine the acute bioenergetic response to transient excitotoxicity and that an AMPK-mediated increase in intracellular glucose may be sufficient to rapidly recover ATP levels following an excitotoxic insult.

Disciplines

Physics | Physiology

Citation

Connolly NM, D'Orsi B, Monsefi N, Huber HJ, Prehn JH1. Computational Analysis of AMPK-Mediated Neuroprotection Suggests Acute Excitotoxic Bioenergetics and Glucose Dynamics Are Regulated by a Minimal Set of Critical Reactions. PLoS One. 2016;11(2):e0148326.

PubMed ID

26840769

DOI Link

10.1371/journal.pone.0148326

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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