Peer Reviewed

1

Document Type

Article

Publication Date

30-4-2015

Keywords

Colorectal cancer, Circulating miRNAs, miR-34a, miR-150, miR-923

Funder/Sponsor

Science Foundation Ireland Strategic Research Cluster, Molecular Therapeutics for Cancer Ireland. Grant Agency of the Czech Republic.

Comments

The original article is available at www.biomedcentral.com

Abstract

BACKGROUND: Screening for the early detection of colorectal cancer is important to improve patient survival. The aim of this study was to investigate the potential of circulating cell-free miRNAs as biomarkers of CRC, and their efficiency at delineating patients with polyps and benign adenomas from normal and cancer patient groups.

METHODS: The expression of 667 miRNAs was assessed in a discovery set of 48 plasma samples comprising normal, polyp, adenoma, and early and advanced cancer samples. Three miRNAs (miR-34a, miR-150, and miR-923) were further examined in a validation cohort of 97 subjects divided into the same five groups, and in an independent public dataset of 40 CRC samples and paired normal tissues.

RESULTS: High levels of circulating miR-34a and low miR-150 levels distinguished groups of patients with polyps from those with advanced cancer (AUC = 0.904), and low circulating miR-150 levels separated patients with adenomas from those with advanced cancer (AUC = 0.875). In addition, the altered expression of miR-34a and miR-150 in an independent public dataset of forty CRC samples and paired normal tissues was confirmed.

CONCLUSION: We identified two circulating miRNAs capable of distinguishing patient groups with different diseases of the colon from each other, and patients with advanced cancer from benign disease groups.

Disciplines

Physics | Physiology

Citation

Aherne ST, Madden SF, Hughes DJ, Pardini B, Naccarati A, Levy M, Vodicka P, Neary P, Dowling P, Clynes M12. Circulating miRNAs miR-34a and miR-150 associated with colorectal cancer progression. BMC Cancer. 2015;15(1):329

PubMed ID

25924769

DOI Link

10.1186/s12885-015-1327-5

Additional File 1.xls (62 kB)
Significantly differentially expressed miRNAs in the independent dataset.

Additional File 2.xls (22 kB)
AUC values for miR-34a, miR-150, and miR-923 in the validation cohort.

Share

COinS