Document Type

Article

Publication Date

7-1-2008

Keywords

Bacterial Adhesion, Bacterial Typing Techniques, Biofilms, Cerebrospinal Fluid Shunts, DNA, Bacterial, Equipment Contamination, Genes, Bacterial, Genotype, Humans, Meningitis, Bacterial, Operon, Polymerase Chain Reaction, Prosthesis-Related Infections, Staphylococcal Infections, Staphylococcus epidermidis

Comments

Published in Clinical Microbiology and Infection 2008 Jul;14(7):719-22. The definitive version is available at www.blackwell-synergy.com

Abstract

Fifty-five Staphylococcus epidermidis isolates, classified as contaminants or causing device-related meningitis, from external ventricular drain (EVD) and non-EVD cerebrospinal fluid specimens were characterized. Thirty-three of 42 (78.6%) meningitis isolates were PCR-positive for ica and aap, known determinants of polysaccharide- and protein-mediated biofilm production, whereas five of 13 (38.5%) contaminants were ica- and aap-negative; 71.4% of meningitis isolates and 84.6% of contaminants produced biofilm. ica+aap+ meningitis isolates produced more biofilm than ica+aap- isolates (p 0.0020). ica+aap- isolates did not produce more biofilm than ica-aap+ isolates (p 0.4368). Apparently, ica and aap are associated with biofilm production in S. epidermidis device-related meningitis isolates.

Disciplines

Medicine and Health Sciences

PubMed ID

18558946

DOI Link

10.1111/j.1469-0691.2008.02012.x