Peer Reviewed

1

Document Type

Article

Publication Date

1-1-2015

Keywords

Base Pairing, Base Sequence, Cell Line, Humans, MicroRNAs, Molecular Sequence Data, Nucleic Acid Hybridization, Oligonucleotides, Antisense, RNA, Messenger

Funder/Sponsor

Science Foundation Ireland

Comments

The final publication is available at Springer via 10.1007/978-1-4939-1538-5_23

Abstract

Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.

Disciplines

Medicine and Health Sciences

Citation

Vencken S, Hassan T, McElvaney NG, Smith SG, Greene CM. miR-CATCH: microRNA capture affinity technology. Methods Molecular Biology. 2015;1218:365-73

PubMed ID

25319664

DOI Link

10.1007/978-1-4939-1538-5_23

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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