Base Pairing, Base Sequence, Cell Line, Humans, MicroRNAs, Molecular Sequence Data, Nucleic Acid Hybridization, Oligonucleotides, Antisense, RNA, Messenger
Science Foundation Ireland
Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.
Medicine and Health Sciences
Vencken S, Hassan T, McElvaney NG, Smith SG, Greene CM. miR-CATCH: microRNA capture affinity technology. Methods Molecular Biology. 2015;1218:365-73
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