Poly(Ethylene glycol)-based backbones with high peptide loading capacities.

Aoife O'Connor, Royal College of Surgeons in Ireland
Jean-Noel Marsat, Royal College of Surgeons in Ireland
Annachiara Mitrugno, Royal College of Surgeons in Ireland
Tom Flahive, Royal College of Surgeons in Ireland
Niamh Moran, Royal College of Surgeons in Ireland
David Brayden, University College Dublin
Marc Devocelle, Royal College of Surgeons in Ireland

Document Type Article

This article is also available at http://www.mdpi.com/1420-3049/19/11/17559

Abstract

Polymer-peptide conjugates are a promising class of compounds, where polymers can be used to overcome some of the limitations associated with peptides intended for therapeutic and/or diagnostic applications. Linear polymers such as poly(ethylene glycol) can be conjugated through terminal moieties and have therefore limited loading capacities. In this research, functionalised linear poly(ethylene glycol)s are utilised for peptide conjugation, to increase their potential loading capacities. These poly(ethylene glycol) derivatives are conjugated to peptide sequences containing representative side-chain functionalised amino acids, using different conjugation chemistries, including copper-catalysed azide-alkyne cycloaddition, amide coupling and thiol-ene reactions. Conjugation of a sequence containing the RGD motif to poly(allyl glycidyl ether) by the thiol-ene reaction, provided a conjugate which could be used in platelet adhesion studies.