Adolescent, Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents, Child, Community-Acquired Infections, Cross Infection, Drug Resistance, Bacterial, Electrophoresis, Gel, Pulsed-Field, Escherichia coli, Escherichia coli Infections, Female, Humans, Ireland, Male, Microbial Sensitivity Tests, Middle Aged, Phylogeny, Young Adult, beta-Lactamases
BACKGROUND: Escherichia coli that produce extended-spectrum beta-lactamases (ESBLs) are an increasing cause of healthcare-associated infection, and community healthcare facilities may be a reservoir for important epidemic clones.
AIM: To characterize retrospectively and investigate the epidemiology of ESBL-producing E. coli collected in a Dublin hospital, during 2009 and 2010, and to investigate the dissemination of specific clones within hospital and community healthcare facilities.
METHODS: Pulsed-field gel electrophoresis (PFGE) was used to determine the genetic relatedness of 100 ESBL-producing E. coli isolates. Phylogenetic groups were determined and the O25b-ST131 clone identified in the collection. The genetic data were correlated with antimicrobial susceptibility, clinical and demographic data to explore the epidemiology of specific clones.
FINDINGS: Phylogenetic groups B2 (62%) and D (18%) were the most common and were associated with non-urinary isolates (P
CONCLUSIONS: E. coli O25b-ST131 is largely responsible for ESBL-producing E. coli in LTCFs in Dublin. The distribution of ESBL-producing E. coli in our hospital and community highlights a 'revolving door' through which these resistant bacteria spread and disseminate.
Medicine and Health Sciences
Burke L, Fitzgerald-Hughes D, Humphreys H. The revolving door between hospital and community: extended-spectrum beta-lactamase-producing Escherichia coli in Dublin. Journal of Hospital Infection. 2012;81(3):192-8.