Peer Reviewed

1

Document Type

Article

Publication Date

14-2-2017

Keywords

Growth Plate, Extracellular Matrix, Scaffold, Large Bone Defect Regeneration, Scaffold Immune Response.

Funder/Sponsor

Funding for this work was provided by AO under the large bone defect healing program, Science Foundation Ireland (12/IA/1554) in addition to the Health Research Board of Ireland (Grant No. HRA_POR/2011/27) and the European Research Council (ERC Grant Agreement No. 239685).

Comments

This article is supplied with kind permission of full reproduction from http://www.ecmjournal.org/journal/papers/vol033/vol033a10.php

Abstract

Limitations associated with demineralised bone matrix and other grafting materials have motivated the development of alternative strategies to enhance the repair of large bone defects. The growth plate (GP) of developing limbs contain a plethora of growth factors and matrix cues which contribute to long bone growth, suggesting that biomaterials derived from its extracellular matrix (ECM) may be uniquely suited to promoting bone regeneration. The goal of this study was to generate porous scaffolds from decellularised GP ECM and to evaluate their ability to enhance host mediated bone regeneration following their implantation into critically-sized rat cranial defects. The scaffolds were first assessed by culturing with primary human macrophages, which demonstrated that decellularisation resulted in reduced IL-1β and IL-8 production. In vitro, GP derived scaffolds were found capable of supporting osteogenesis of mesenchymal stem cells via either an intramembranous or an endochondral pathway, demonstrating the intrinsic osteoinductivity of the biomaterial. Furthermore, upon implantation into cranial defects, GP derived scaffolds were observed to accelerate vessel in-growth, mineralisation and de novo bone formation. These results support the use of decellularised GP ECM as a scaffold for large bone defect regeneration.

Disciplines

Anatomy

Citation

Cunniffe GM, Díaz-Payno PJ, Ramey JS, Mahon OR, Dunne A, Thompson EM, O'Brien FJ, Kelly DJ. Growth plate extracellular matrix-derived scaffolds for large bone defect healing. European Cells & Materials. 2017;33:130-142.

PubMed ID

28197989

DOI Link

10.22203/eCM.v033a10

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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Anatomy Commons

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