Document Type

Article

Publication Date

1-4-2009

Keywords

Animals, Biomechanics, Bone and Bones, Cattle, Cell Count, Cells, Cultured, Collagen, Compressive Strength, Cross-Linking Reagents, Elastic Modulus, Glycosaminoglycans, Permeability, Porosity, Tissue Engineering, Tissue Scaffolds

Comments

The original publication is available at http://www.sciencedirect.com

Abstract

In this study, we examined the effects of varying collagen concentration and crosslink density on the biological, structural and mechanical properties of collagen-GAG scaffolds for bone tissue engineering. Three different collagen contents (0.25%, 0.5% and 1% collagen) and two different dehydrothermal (DHT) crosslinking processes [1] 105 degrees C for 24 h and [2] 150 degrees C for 48 h were investigated. These scaffolds were assessed for (1) pore size, (2) permeability (3) compressive strength and (4) cell viability. The largest pore size, permeability rate, compressive modulus, cell number and cell metabolic activity was all found to occur on the 1% collagen scaffold due to its increased collagen composition and the DHT treatment at 150 degrees C was found to significantly improve the mechanical properties and not to affect cellular number or metabolic activity. These results indicate that doubling the collagen content to 1% and dehydrothermally crosslinking the scaffold at 150 degrees C for 48 h has enhanced mechanical and biological properties of the scaffold making it highly attractive for use in bone tissue engineering.

Disciplines

Anatomy

Citation

Tierney CM, Haugh MG, Liedl J, Mulcahy F, Hayes B, O'Brien FJ. The effects of collagen concentration and crosslink density on the biological, structural and mechanical properties of collagen-GAG scaffolds for bone tissue engineering. Journal of the Mechanical Behavior of Biomedical Materials 2009;2(2):202-9.

PubMed ID

19627824

DOI Link

10.1016/j.jmbbm.2008.08.007

Included in

Anatomy Commons

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