Document Type

Article

Publication Date

1-2-2015

Keywords

Scaffolds, Mean Pore Size, Mesenchymal Stem Cells, Chondrogenesis, Collagen

Funder/Sponsor

Enterprise Ireland, Proof of Concept PC/2007/331, Commercialisation Fund Technology Development Phase CFTD/2009/0104 and Health Research Board of Ireland HRA_POR/2011/27. We also acknowledge the support of Dr. Clodagh Dooley, Centre for Microscopy and Analysis, TCD, Dublin for providing SEM imaging support.

Abstract

Recent investigations into micro-architecture of scaffolds has revealed that mean pore sizes are cell-type specific and influence cellular shape, differentiation, and extracellular matrix secretion. In this context, the overall goal of this study was to investigate whether scaffold mean pore sizes affect mesenchymal stem cell initial attachment, chondrogenic gene expression, and cartilage-like matrix deposition. Collagen-hyaluronic acid (CHyA) scaffolds, recently developed in our laboratory for in vitro chondrogenesis, were fabricated with three distinct mean pore sizes (94, 130, and 300 μm) by altering the freeze-drying technique used. It was evident that scaffolds with the largest mean pore sizes (300 μm) stimulated significantly higher cell proliferation, chondrogenic gene expression, cartilage-like matrix deposition, and resulting bulk compressive modulus after in vitro culture, relative to scaffolds with smaller mean pore sizes (94, 130 μm). Taken together, these findings demonstrate the importance of scaffold micro-architecture in the development of advanced tissue engineering strategies for articular cartilage defect repair.

Disciplines

Anatomy

Citation

Matsiko A, Gleeson J, O'Brien FJ. Scaffold mean pore size influences mesenchymal stem cell chondrogenic differentiation and matrix deposition. Tissue Engineering. Part A. 2015;21(3-4):486-97.

PubMed ID

25203687

DOI Link

10.1089/ten.TEA.2013.0545

Creative Commons License


This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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Anatomy Commons

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