Peer Reviewed

1

Document Type

Article

Publication Date

1-8-2011

Keywords

Biological Markers, Blood Proteins, Chromatography, Liquid, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Humans, Immunoglobulins, Proteomics

Comments

The original article is available at www.wiley.com

Abstract

Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2-DE. With alteration of the manufacturer protocol, the Seppro(®) IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2-DE compared to untreated serum.

Disciplines

Anatomy

Citation

Corrigan L, Jefferies C, Clive Lee T, Daly J. Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum. Proteomics. 2011;11(16):3415-9.

PubMed ID

21751350

DOI Link

10.1002/pmic.201000587

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